University of California - San Diego #92496 Tumor Biology Lab Research Assistant in San Diego, California
#92496 Tumor Biology Lab Research Assistant
Filing Deadline: Mon 3/26/2018
UCSD Layoff from Career Appointment: Apply by 3/15/18 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Eligible Disability Counseling and Consulting services (DCC) or Special Selection clients should contact their Vocational Rehabilitation Counselor for assistance.
Under supervision, perform cell culture and propagation of tumor cell lines as well as transfection and cellular fractionation experiments. Independently maintain tumor cell and organoid cultures for use in transfection and infection experiments. Responsible for maintaining and inventory of frozen cell stocks and routine testing for mycoplasma contamination. Process tumor specimens by extracting DNA and RNA, as well as perform cloning and subcloning of DNA fragments. Perform molecular biology/cell biologic assays, to study mouse models of cancer. Works with animal models of cancer. Perform animal crosses and genotyping, and tumor formation assays by transplantation of cancer cells into mice. Perform computational analysis and data management. Perform ordering, inventory, and basic laboratory supply management. Act as the Area Safety Coordinator for assigned laboratory space. Responsible for EH&S compliance of the laboratory and the required training of lab personnel. Prepare chemical, biological, and radiation protocols for submission to EH&S committees for review. Assist Principal Investigator with departmental budgetary and personnel matters. Integrate information obtained from scientific journals for use in the laboratory and disseminate this information to other lab members.
Theoretical knowledge of cell biology and molecular biology as typically attained by a Bachelor's degree in Biology, Chemistry, or other related areas, and/or equivalent education and experience.
Experience performing routine cell culture techniques, such as pipetting culture media onto growing human cells in culture plates and pipetting trypsin solution onto cell layers. Experience following basic research protocols and instructions closely.
Experience working with mice for surgical procedures, including anesthesia administration. Experience preparing reagents and cells for animal studies, performing in vivo luciferase assays, and measurement of tumor size.
Experience with extracting DNA and RNA from tumor specimens, and cloning and subcloning of pertinent DNA fragments. Experience performing PCR and qPCR reactions.
Experience with preparing chemical solutions, including performing gel electrophoresis by mixing gel solutions and pouring into gel molds. Ability to perform standard mathematic computations (calculate molarity) accurately.
Basic knowledge of Computational Biology and Bioinformatics.
Knowledge of laboratory procedures and equipment, such as sterile techniques, preparation of buffers, centrifuges, balance pH meters and electrophoresis equipment.
- Knowledge of computational biology including specifically the use of cancer genomics tools and applications.
- Employment is subject to a criminal background check.
UC San Diego Health is the only academic health system in the San Diego region, providing leading-edge care in patient care, biomedical research, education, and community service. Our facilities include two university hospitals, a National Cancer Institute-designated Comprehensive Cancer Center, Shiley Eye Institute, Sulpizio Cardiovascular Center, and several outpatient clinics. UC San Diego Medical Center in Hillcrest is a designated Level I Trauma Center and has the only Burn Center in the county. We invite you to join our dynamic team!
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UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html
UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.
SRA 2 NEX
CANCER CENTER/Solid Tumor Therapeutics
Commensurate with Experience
Moores Cancer Center
Days, 8 hrs/day, Mon-Fri
As a federally-funded institution, UC San Diego Health maintains a marijuana and drug free campus. New employees are subject to drug screening.